A new role for the brain chemical dopamine that is independent of classic neurotransmission has been discovered by scientists. The new role seems to be vital to changes in gene expression connected to chronic exposure to, or abuse of, cocaine.
The novel role for the brain chemical dopamine that is independent of classic neurotransmission was discovered by scientists at the Icahn School of Medicine at Mount Sinai . It seems to be vital to changes in gene expression connected to chronic exposure to, or abuse of, cocaine, according to a study published in the journal Science.
Ian Maze, PhD, Associate Professor of Neuroscience, and Pharmacological Sciences, at the Icahn School of Medicine at Mount Sinai, and lead author of the study. says “Our study provides the first evidence of how dopamine can directly impact drug-induced gene expression abnormalities and subsequent relapse behavior.” “We have found that dopamine can be chemically attached to histone proteins, which causes cells to switch different genes on and off, affecting regions of the brain that are involved in motivation and reward behavior, beyond transmission of signals between neurons in the brain. When perturbed by drugs of abuse, this biochemical process significantly affects cocaine vulnerability and relapse.”
DNA and how it works to form each person’s individual biological map is what the study revolves around. Two meters of DNA, the blueprint for all functions of all cells in the body is contained in each cell of the body. Spools of histone proteins (proteins that package DNA in the nucleus of cells, and are heavily prone to chemical modifications that aid in the regulation of gene expression) into structures referred to as nucleosomes, is where this DNA is wound around. That gene is less likely to be expressed when DNA encoding a specific gene is wound tightly within the spool. It is more likely to be expressed when the gene is not wound as tightly. A given cells many functions can be affected by this.
Dopamine is a chemical that ferries information between neurons or also known as the feel-good neurotransmitter. The brain releases it when we eat food that we crave or while we have sex, contributing to feelings of pleasure and satisfaction as part of the natural reward system. This important neurochemical boosts mood, motivation, and attention, and helps regulate movement, learning, and emotional responses. Dopamine also enables us not only to see rewards but to take action to move toward them.
Vulnerability to relapse during periods of cocaine withdrawal is believed to result from functional rewiring of the brain’s reward circuitry, particularly within mid-brain regions, such as the ventral tegmental area (VTA). The research team discovered that a protein called transglutaminase 2 can directly attach dopamine molecules to histone proteins (a process called histone dopaminylation or H3Q5dop) which, in turn, affects the histone-DNA spool to enable environmentally regulated alterations in gene expression. They found that histone dopaminylation plays a critical role in fueling heightened vulnerability to relapse over a prolonged period of time. Specifically, accumulation of H3Q5dop in the VTA can, in effect, hijack the reward circuitry, making it difficult to distinguish between good and maladaptive behavior. The study found, however, that reducing H3Q5dop in rats programmed to undergo withdrawal from cocaine significantly reversed cocaine-mediated gene expression changes and reduced cocaine-seeking behavior.
“The question that has always challenged neuroscientists is, what are the underlying molecular phenomena that drive increased vulnerability to drug relapse in people,” says Ashley Lepack, PhD, a researcher in the Department of Neuroscience, The Friedman Brain Institute, in Dr. Maze’s lab at Mount Sinai, and first author of the study. “Our research is shedding valuable light on this area by identifying histone dopaminylation as a new, neurotransmission-independent role for dopamine that hasn’t been implicated before in brain pathology.”
“We believe these findings represent a paradigm shift in how we think of dopamine, not just in the context of drug abuse, but also potentially in other reward-related behaviors and disorders, as well as in neurodegenerative diseases like Parkinson’s, where dopamine neurons are dying,” says Dr. Maze. “In this case, the question becomes, ‘could this neuronal death be due, in part, to aberrant dopaminylation of histone proteins?’ ”
In a study published last year, Dr. Maze and his team found that another neurotransmitter, serotonin, a chemical involved in the regulation of mood, acts in a similar way as dopamine on gene expression inside brain cells.
“When we observed this unique signaling mechanism with serotonin, we decided to look at other neurotransmitters, particularly dopamine, and found that it could also undergo this type of chemical modification on the same histone protein,” explains Dr. Maze.
Early-stage work with human post-mortem tissues has demonstrated to Dr. Maze that strong parallels may well exist, but that basic questions around biochemical function still remain before human trials can begin. “From a therapeutic standpoint, we’ve started to identify from rodent models the mechanisms that can actually reverse aberrant and addictive behaviors,” says Dr. Maze, “and that knowledge could be vital to moving this novel research into the clinic.”
Source: Mount Sinai